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Comparative metagenome of a stream impacted by the urbanization phenomenon BJM
Medeiros,Julliane Dutra; Cantão,Maurício Egídio; Cesar,Dionéia Evangelista; Nicolás,Marisa Fabiana; Diniz,Cláudio Galuppo; Silva,Vânia Lúcia; Vasconcelos,Ana Tereza Ribeiro de; Coelho,Cíntia Marques.
Abstract Rivers and streams are important reservoirs of freshwater for human consumption. These ecosystems are threatened by increasing urbanization, because raw sewage discharged into them alters their nutrient content and may affect the composition of their microbial community. In the present study, we investigate the taxonomic and functional profile of the microbial community in an urban lotic environment. Samples of running water were collected at two points in the São Pedro stream: an upstream preserved and non-urbanized area, and a polluted urbanized area with discharged sewage. The metagenomic DNA was sequenced by pyrosequencing. Differences were observed in the community composition at the two sites. The non-urbanized area was overrepresented by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquatic environment; Urbanization; Microbial community; Metagenomic.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400835
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Darcy Fontoura de Almeida (1930-2014) Genet. Mol. Biol.
Vasconcelos,Ana Tereza Ribeiro de; Costa,Sergio Olavo Pinto da; Goldenberg,Samuel; Ferreira,Luís Carlos de Souza.
Tipo: Info:eu-repo/semantics/article
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300002
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Genes involved in cell division in mycoplasmas Genet. Mol. Biol.
Alarcón,Frank; Vasconcelos,Ana Tereza Ribeiro de; Yim,Lucia; Zaha,Arnaldo.
Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw) cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell division; Mycoplasma spp; Genomes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200003
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In search of essentiality: Mollicute-specific genes shared by twelve genomes Genet. Mol. Biol.
Souza,Rangel Celso; Almeida,Darcy Fontoura de; Zaha,Arnaldo; Morais,David Anderson de Lima; Vasconcelos,Ana Tereza Ribeiro de.
Mollicutes are cell wall-less bacteria with a genome characterized by its small size. Chromosomal rearrangements help these organisms evade host immune surveillance and hence cause disease. Our goal was to determine genes shared by Mollicutes genomes using the bidirectional best hit methodology. The twelve studied Mollicutes share 210 genes, most of which (> 60%) fall into the following COG categories: translation, ribosomal structure and biogenesis; DNA replication, recombination and repair; nucleotide transport and metabolism and energy production and conversion. Thirty Mollicute-specific genes were identified, 22 of them previously described as essential genes in Mycoplasma genitalium.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Comparative genomics; Synteny; Bidirectional best hit.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200002
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Restriction-modification systems in Mycoplasma spp Genet. Mol. Biol.
Brocchi,Marcelo; Vasconcelos,Ana Tereza Ribeiro de; Zaha,Arnaldo.
Restriction and Modification (R-M) systems are present in all Mycoplasma species sequenced so far. The presence of these genes poses barriers to gene transfer and could protect the cell against phage infections. The number and types of R-M genes between different Mycoplasma species are variable, which is characteristic of a polymorphism. The majority of the CDSs code for Type III R-M systems and particularly for methyltransferase enzymes, which suggests that functions other than the protection against the invasion of heterologous DNA may exist. A possible function of these enzymes could be the protection against the invasion of other but similar R-M systems. In Mycoplasma hyopneumoniae strain J, three of the putative methyltransferase genes were clustered...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Restriction-modification systems; Mycoplasma spp; Genomes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200011
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